Wide antimicrobial activity

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Proven biocidal activity against planktonic bacteria, amoeba and realistic biofilms

Planktonic and sessile bacteria

The minimum killing effect of our Green biocide/BIOMEBA was tested as for antibiogram testing. Results showed a phagocytotic plaque after addition of our biological biocide, effective against all the bacterial strains in the following table. In addition, Green biocide/BIOMEBA was demonstrated to perform better than all chemical biocides during a 2-year field test period in several companies, with daily monitoring of its effect on Legionella.

Organism

ATCC

Active concentration

(106 cells/l)

Staphylococcus aureus

6538

5

Legionella pneumophila paris

Env*

5

Legionella pneumophila philadelphia

33152

5

Legionella pneumophila Fraseri

33156

5

Legionella spp

Env*

5

Listeria monocytogenes**

7644

5

Klebsiella pneumonia

4352

5

Aeromonas hydrophila

7966

5

Streptococcus spp

9854

5

E.coli

11229

5

Myroides odoratus

4651

5

Pseudomonas aeruginosa**

10145

5

Salmonella enterica sv typhimurium

14028

5

Pseudomonas aeruginosa**

27853

5

 *Env= harvested from the environment
** Amoéba has lodged a patent application relating to use of its product with these microorganisms

For some organisms, the current regulations, in particular regulation EN 13623 with reference to the Legionella strains, defines the conditions in which efficiency tests should be performed. These conditions were applied during our tests.

Amoeba (replicative forms and encysted forms)

Amoéba's Green biocide/BIOMEBA has been confirmed to be effective against the following amoebas, whether they are in replicative or cystic forms.

Organism

ATCC

Active concentration (106 cells/l)

Hartmannella vermiformis

Env*

5

Acanthamoeba castellani

30010

5

Naegleria fowleri

30809

5

 *Env= harvested from the environment

Realistic biofilms

In just 24 hours, a single application of our biological biocide can decrease the total viable count on a mature biofilm composed of 4 bacteria - including Legionella pneumophila - by 99%.

Moreover, a single dose prevents the reformation of the biofilm over the following 4 days.

Amoéba wished to test the limits of efficacy for its Green biocide/BIOMEBA in this application, since it is generally recognised that 95% of pathogenic bacteria are included in biofilms. Because of this observation, an effective biocide must kill sessile pathogenic bacteria present in the biofilm, but it must also reduce the total viable count in highly realistic biofilms.

Amoéba has been extremely thorough in validating its biocide's activity against biofilms. Our researchers, in collaboration with worldwide biofilm specialists, have designed a highly realistic mature biofilm, composed of at least 4 bacteria and amoeba.

Mature biofilm is formed by incubation and growth for more than 7 days in culture media. In contrast, an early biofilm consists of a pre-grown biofilm less than 48-hours-old.

The inclusion of multiple bacteria is mandatory because the interaction between the various bacterial strains leads to an inter-bacterial mechanism of resistance.

The presence of amoebas was also tested, since amoebas play a key role in the acquisition of bacterial resistance to chemical biocides. (1 ppm of CMIT/MIT is required to inhibit the development of free legionella; whereas 16 ppm of CMIT/MIT is required to inhibit the same legionella concentration after pre-incubation with amoebas).