Ecological testing

You are here

A total absence of ecotoxicity

Amoéba's biocide BIOMEBA has demonstrated its absence of negative environmental impact through the following performed ecotoxicity studies:

Daphnia magna, Acute Immobilization Test

Study No. 10-010-116210 based on OECD Test No. 202: Daphnia sp. Acute Immobilization Test. Results: The absence of inhibitory effect during the limit test demonstrates the EC50 of Willaertia Magna C2c Maky on Daphnia magna is greater than 100.106 amoeba/L and so greater than the maximum recommended concentration of use (106 amoeba/L) after 48hours.

Fish, Acute Toxicity Test

Study No. 10-011-116210 based on OECD Test No. 203: Fish, Acute Toxicity Test. Results:  The absence of mortality during this limit test demonstrates that the LC50 of the test Willaertia Magna C2c Maky on Danio rerio is greater than 71.8 106 amoeba/L and also the maximum recommended concentration of use (106 amoeba/L) after 96 hours of exposure.

Alga, Growth Inhibition Test

Study No. 10-009-116210 based on OECD Test No. 201: Freshwater Alga and Cyanobacteria, Growth Inhibition Test. Results:  The absence of inhibitory effect during this limit test demonstrates that the EC50 of Willaertia Magna C2c Maky on the growth of the unicellular green alga species: Pseudokirchneriella subcapitata is greater than 60.106 amoeba/l and also the maximum recommended concentration of use (106 amoeba/L) after 72 hours of exposure.

Activated Sludge, Respiration Inhibition Test

Study No. 11-003-116210 based on OECD Test No. 209: Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation). Results: The absence of inhibitory effect during this limit test demonstrates that the EC50 of the test item, on respiration of activated sludge, is greater than 100.106 amoebae/L (initial concentration), after a contact time of 3 hours.

Earthworm acute toxicity study

Study No. 15-004-155072 based on OECD Test No. 209: Earthworm, Acute Toxicity Test. Results:  No effect of the test item on the survival of the earthworms Eisenia fetida was measured at the test concentration of 2,71.109 amoeba/kg of soil (dry weight). It should be noticed a significant increase of biomass of the earthworms exposed to amoeba, during 14 days, compared to unfed control (36.1% versus 7.9% for control conditions).

Acute contact toxicity on honey bees (Apis mellifera)

Study No. 15/201-216MT based on OECD Test No. 214: Honeybees, Acute Contact Toxicity Test. The acute contact toxicity of the living test item (Willaertia magna C2c Maky) was tested on honey bees (Apis mellifera L.) under laboratory conditions. Based on the results the test item is not toxic to honey bees at the maximal commercial concentration of Amoéba biocide (~ 3 x 109 cells/L/bee). No adverse effects were noticed on behaviour.

Chronic oral toxicity study on honey bees (Apis mellifera)

Study No. 15/201-216MT based on OECD Test No. 213: Honeybees - Acute Oral Toxicity Test, EFSA Guidance on risk assessment on Bees (4 July 2013) and US EPA Microbial Pesticide Test Guidelines - OPPTS 885.4380 Honey Bee Testing,Tier I. Results:  The toxicity of the living test item (Willaertia magna C2c Maky) was tested on honey bees (Apis mellifera) in a chronic oral toxicity study under laboratory conditions. Based on the results the test item is not toxic to honey bees at the maximal commercial concentration of Amoéba biocide (3 x 109 cells/L). No adverse effects were noticed on behaviour.

Soil Microorganisms: Carbon Transformation Test

Study No. 402/15/1153F/d-e based on OECD Test No. 217: Soil Microorganisms: Carbon Transformation Test. Results:  test item had no long-term adverse effect on carbon transformation because no impact of Willaertia magna C2c Maky was recorded on respiration rate.

Soil Microorganisms: Nitrogen Transformation Test

Study No. 402/15/1153F/c-e based on OECD Test No. 216: Soil Microorganisms: Nitrogen Transformation Test. Results:  A growth of nitrate content was observed in MEM Control and test item containers relative to the control. So, no inhibition of nitrate production was observed, but an increase of the nitrate content has been found in the test item and the MEM Control compared to the Control. The presence of Willaertia magna C2c Maky showed no inhibition on the nitrate production.

Effects on seedling emergence and early growth of higher terrestrial plants

Study No. 402/15/1153F/b-e based on OECD Test No. 208: Terrestrial Plant Test: Seedling Emergence and Seedling Growth Test. Results:  the test item Willaertia magna C2c Maky has no effect on germination and on results of biomass.

Toxicity to freshwater aquatic plants

Study No. 15-006-155072 based on OECD Test No. 221: Lemna sp. Growth Inhibition Test. Results: The study results showed that under the test conditions the test item: Willaertia magna C2c Maky has no significant toxic effect on vegetative growth of Lemna minor to the initial concentration of 3.0E+09 amoeba/L (Time-weighted mean over 7 days: 1.74E+09 amoeba/L).

Acute oral toxicity of substances to birds

Study No. ST042 based on OECD Test No. 223: Avian Acute Oral Toxicity Test. Results: As no mortality on the birds was observed in the limit dose test like 0n the control birds, the DL50 value for the oral administration of Willaertia magna C2c Maky in the Japanes quail (Coturnix coturnix japonica) is higher than 2000 mg/kg of body weight.